Synthetic 5-amino-6-D-ribitylaminouracil paired with inflammatory stimuli facilitates MAIT cell expansion in vivo

AG Nelson; H Wang; PM Dewar; EM Eddy; S Li; XY Lim; T Patton; Y Zhou; TJ Pediongco; LJ Meehan; BS Meehan; JYW Mak; DP Fairlie; AW Stent; L Kjer-Nielsen; J McCluskey; SBG Eckle; AJ Corbett; MNT Souter; Z Chen

Journal article

Synthetic 5-amino-6-D-ribitylaminouracil paired with inflammatory stimuli facilitates MAIT cell expansion in vivo

AG Nelson, H Wang, PM Dewar, EM Eddy, S Li, XY Lim, T Patton, Y Zhou, TJ Pediongco, LJ Meehan, BS Meehan, JYW Mak, DP Fairlie, AW Stent, L Kjer-Nielsen, J McCluskey, SBG Eckle, AJ Corbett, MNT Souter, Z Chen

Frontiers in Immunology | Published : 2023

DOI: 10.3389/fimmu.2023.1109759

Abstract

Introduction: Mucosal-associated invariant T (MAIT) cells are a population of innate-like T cells, which mediate host immunity to microbial infection by recognizing metabolite antigens derived from microbial riboflavin synthesis presented by the MHC-I-related protein 1 (MR1). Namely, the potent MAIT cell antigens, 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU) and 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU), form via the condensation of the riboflavin precursor 5-amino-6-D-ribitylaminouracil (5-A-RU) with the reactive carbonyl species (RCS) methylglyoxal (MG) and glyoxal (G), respectively. Although MAIT cells are abundant in humans, they are rare in mice, and increa..

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University of Melbourne Researchers

Huimeng Wang Author

Timothy Patton Author

Lucy Meehan Author

Bronwyn Meehan Author

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Grants

Awarded by Guangzhou Medical University

Funding Acknowledgements

This work was supported by grants from the National Health and Medical Research Council of Australia (NHMRC) (1113293, 1120467) and the U.S. National Institute of Health (RO1 Grant AI148407-01A1). SE and AC are supported by Dame Kate Campbell Fellowships from the University of Melbourne. JMc, AC, DF and SE are supported by NHMRC Investigator grants (JM; 2008616, DF 2009551, AC: 1193745 and SE: 1196881). HW is supported by the National Natural Science Foundation of China Young Scientists Fund (82001686) and the Guangzhou Medical University Nanshan Fellowship.